Hematologic assessed of B6.GT mice. (A) Peripheral blood hematology analysis of mouse blood collected from individual B6.WT (n = 7), B6.TRAIL^−/− (n = 8), B6.gld/gld (n = 16), and B6.GT (n = 15) mice, more than 20 weeks of age. Blood was collected from freshly killed mice directly from the inferior vena cava into 5 mL potassium K₂-EDTA acid-coated blood collection tubes and analyzed within 4 hours of collection on an ADIVA120 Hematology System analyzer. Each symbol differentiates an individual mouse across all hematologic parameters assessed. The normal range of each parameter in mouse blood is shown (dashed lines). (B) Lung histology. Hematoxylin and eosin staining of formalin-fixed paraffin-embedded lung tissues. Shown are sections of lung tissue from 1 representative B6.WT, B6.TRAIL^−/−, B6.gld/gld, and B6.GT mouse, and data are representative of repeated analysis of independently generated mouse cohorts. (C) Assessment of T cell and DN T cell clonality as determined by conventional RT-PCR of Vβ-specific TCR CDR3 mRNAs, using RNA obtained from mouse bone marrow (BM), axillary lymph node (LN), and peripheral blood leukocytes (PBL) from a representative mouse 20 weeks of age or older from each strain, or from 7 B6.GT mice. Broad clonality was confirmed using spectratyping analysis on each PCR product. Spectratyping data shown are from PBL TCR Vβ-specific RT-PCR products, with the correlating total white cell counts for the individual mouse, as indicated.