NuSAP1 and CHAF1b are highly expressed in CD34⁺CD90⁺HSCs as demonstrated by RT-PCR quantification. One bone marrow biopsy was sorted by flow cytometry into CD34⁺, CD34⁻, CD34⁺CD90⁺, and CD34⁺CD90⁻ subpopulations and screened for NuSAP1 (A) and CHAF1b (B) expression. A total of 12 AML PBMNC samples and GM-CSF–mobilized peripheral blood samples (n = 5) were screened, and these samples were further sorted into CD34⁺, CD34⁻, CD34⁺CD90⁺ and CD34⁺CD90⁻, CD15, CD14, CD4, CD8, CD19, and dendritic cell subpopulations. DNA was obtained from all subpopulations and reverse-transcribed to cDNA using OligodT primers. cDNA was screened for NuSAP1 and CHAF1b expression using the respective specific primers using RT-PCR. Results obtained by RT-PCR were plotted relative to the positive control gene β-actin. Error bars indicate SE of measurement.