Tmprss6^−/−Hjv^−/− and Tmprss6^+/+Hjv^−/− mice show similar iron distribution in liver and spleen. Perls Prussian blue staining of histologic sections of liver (A-D) and spleen (E-H). Blue staining represents iron accumulation in cells. Tmprss6^+/+Hjv^+/+ (A) and Tmprss6^−/−Hjv^+/+ (B) mice lack histologic evidence of excess iron deposition, whereas Tmprss6^+/+Hjv^−/− (C) and Tmprss6^−/−Hjv^−/− (D) mice show marked iron accumulation in hepatocytes. Splenic macrophages of Tmprss6^+/+Hjv^+/+ (E) and Tmprss6^−/−Hjv^+/+ (F) mice show abundant iron, whereas minimal iron is detected in splenic macrophages of Tmprss6^+/+Hjv^−/− (G) and Tmprss6^−/−Hjv^−/− (H) mice. Original magnification ×200 (A-D) and ×100 (E-H).