Figure 5
![Figure 5. E-selectin engagement induces phosphorylation of Btk, PLCγ2, and PI3K. Bone marrow–derived neutrophils were plated on uncoated (unstimulated) or E-selectin–coated wells for 10 minutes, and then lysates were prepared. (A) Lysates were immunoprecipitated with anti-Btk, followed by immunoblotting (IB) with a general phosphotyrosine (PY; 4G10) antibody. (B) Lysates were immunoblotted with antibody to phosphorylated PLCγ2 (phospho-PLCγ2 [Tyr1217]), total PLCγ2 (n = 3), phosphorylated Akt (n = 3), total Akt (n = 3), phosphorylated p38 MAPK (phospho-p38), or total p38 (n = 3).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/115/15/10.1182_blood-2009-11-254185/4/zh89991051370005.jpeg?Expires=1735508385&Signature=iP2FNzJSRC87BVspupAMNfZTV8VOuq~uLni~EBy1e5RJ7Xd~huEz3lga1r0TNckxiZNQbTR9e56bQsCMOX1vORQ047im1U0FJptOaLTuXrRODGpSQB7P6szzm8tkdflPUtdOumbMVg3RW6i-gZkuVlMftjojHzOJoIHLYsylYhHTQuxWjQkGGN6O3kIhpv1p4UR7XeVCqujvDmMiN8Vz9mcnzVqnXmpOucGV3F4lwn-xqA3CsDJ9liudykOED5H2wYON7EKXHrS1iHIySyPM~MFTsXQpU1UuQEQm45yKn4CCiHqLMJkgrt1DCdzvhZTIx-3sCscLwbj7EJvsJpsKWg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
E-selectin engagement induces phosphorylation of Btk, PLCγ2, and PI3K. Bone marrow–derived neutrophils were plated on uncoated (unstimulated) or E-selectin–coated wells for 10 minutes, and then lysates were prepared. (A) Lysates were immunoprecipitated with anti-Btk, followed by immunoblotting (IB) with a general phosphotyrosine (PY; 4G10) antibody. (B) Lysates were immunoblotted with antibody to phosphorylated PLCγ2 (phospho-PLCγ2 [Tyr1217]), total PLCγ2 (n = 3), phosphorylated Akt (n = 3), total Akt (n = 3), phosphorylated p38 MAPK (phospho-p38), or total p38 (n = 3).
