15(S)-HETE induces FGF-2 expression rapidly in HDMVECs. (A-B) Quiescent HDMVECs were treated with and without 15(S)-HETE (0.1μM) for various time periods, and either RNA was isolated or cell extracts were prepared. RNA was analyzed by QRT-PCR for FGF-2 and β-actin mRNA levels, and cell extracts were analyzed by Western blotting for FGF-2 levels with the use of its specific antibodies. The blot was reprobed with anti–β-tubulin antibodies for normalization. (C) All the conditions were the same as in panel B except that medium was collected and analyzed for FGF-2 release by ELISA. The bar graphs in panels A and C represent the quantitative analysis of 3 independent experiments. The values are presented as the means ± SD. *P < .01 vs control.