Effect of rh-sCD146 on angiogenic capacity of EPCs in vitro. (A) EPC capacity to elaborate pseudocapillaries in Matrigel plugs was evaluated in different conditions (control, rh-sCD146, c-myc peptide, immunodepleted rh-sCD146 [Ip rh-sCD146] and its control [IpC], or VEGF). Number of tubes was counted after 5 hours of incubation. Results are the mean ± SEM values of 6 different experiments. (B) Proliferation capacity of late EPCs was evaluated as described in “Cell proliferation assay.” Results are the mean plus or minus SEM values of 5 different experiments. (C) Migration capacity of late EPCs was evaluated using a wound healing assay. Results are the mean ± SEM values of 4 different experiments. *P < .05, **P < .01, ***P < .001, experimental versus control.