Analysis of transformation ability of various AML1-ETO point mutants in murine primary hematopoietic cells. (A) AML1-ETO point mutants used in the retroviral transduction/transformation assay are indicated on the left. The bar chart (right) represents the numbers of colonies after each plating in methylcellulose. Error bars show standard deviations of indicated numbers (n) of independent experiments. (B) Typical third-round colony morphology of primary transduced bone marrow cells transduced with indicated constructs. The top panel shows colonies stained with INT and the bottom panel shows unstained colonies (×10 magnification). (C) Phenotypical analysis of cells transformed by indicated constructs. Dot plots represent stainings obtained with antibodies specific for the indicated surface markers. Contour plots show unstained controls.