Neogenin expression in mouse liver. (A) Detection of enzymatic lacZ activity in livers from P25-old neogenin^m/m mice. (B) Illustration of zonal distribution of neogenin protein in hepatocytes close to central veins and nonhepatocytes align with portal vein (green color). (C) Immunohistochemical detection of neogenin in livers from P25 neogenin^+/+ and neogenin^m/m mice. (D) Confocal images of coimmunohistochemical analysis in liver sections of P25 wild-type mouse using antibodies against neogenin (polyclonal, red) and GS (monoclonal, green). Arrows point to neogenin-expressing hepatocytes. CV indicates central vein; and PT, portal tract. Bars represent 100 μm. In panels A, C, and D, 3 to 5 different mice for each indicated group were examined, and representative images are shown.