Neogenin inhibition of HJV secretion. (A-B) Neogenin inhibition of HJV secretion in serum-starved culture. Transfected HEK293 cells were cultured in the presence of 0.5% of serum and the cell lysates and medium were subjected to Western analysis (A). The HJV secretion in the collected medium was also measured by ELISA analysis (B). The mean ± SEM of HJV secretion (binding to the antibodies) was presented. *P < .01, significant difference from the HJV expression alone. (C) Illustration of P15 muscle culture and Western blot analysis of cell lysates and medium from wild-type and neogenin mutant muscle culture with indicated antibodies. Note that HJV was increased in the medium, but reduced in cell lysates, of neogenin mutant culture. (D) Quantification analysis of data from panel C. The data were quantified by National Institutes of Health ImageJ program from 3 different blots and presented as means ± SD. *P < .01, significant difference from the wild-type control.