American Society of Hematology

Figure 3

$Figure 3. Integrin or chemokine receptor engagement on human NK cells enhances WASp tyrosine phosphorylation status. Human NK cells were left untreated (-) or incubated with GAM cross-linked anti-β1 (TS2/16), anti-β2 (TS1/18), anti-α4 (HP2/1), or anti-CD56 (C218) mAb (A) or stimulated with CXCL12/SDF-1, CX3CL1/fractalkine (B) for the indicated time periods at 37°C. Cell lysates were then immunoprecipitated with anti-WASp mAb. The resulting immunocomplexes were resolved by 7.5% sodium dodecyl sulfate–polyacrylamide gel electrophoresis, transferred to nitrocellulose, and sequentially immunoblotted with anti-pTyr mAb (top) and anti-WASp antiserum (bottom). Sizes are indicated in kilodaltons. These results represent 1 of 3 independent experiments.$

Integrin or chemokine receptor engagement on human NK cells enhances WASp tyrosine phosphorylation status. Human NK cells were left untreated (-) or incubated with GAM cross-linked anti-β1 (TS2/16), anti-β2 (TS1/18), anti-α4 (HP2/1), or anti-CD56 (C218) mAb (A) or stimulated with CXCL12/SDF-1, CX3CL1/fractalkine (B) for the indicated time periods at 37°C. Cell lysates were then immunoprecipitated with anti-WASp mAb. The resulting immunocomplexes were resolved by 7.5% sodium dodecyl sulfate–polyacrylamide gel electrophoresis, transferred to nitrocellulose, and sequentially immunoblotted with anti-pTyr mAb (top) and anti-WASp antiserum (bottom). Sizes are indicated in kilodaltons. These results represent 1 of 3 independent experiments.

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