American Society of Hematology

Figure 6

$Figure 6. The ability of chemokine to induce LFA-1 high-affinity state is impaired in NK cells from patients with WAS. Freshly isolated peripheral blood NK cells from healthy donors or patients with WAS were left untreated or stimulated with CXCL12/SDF-1 (10nM) or CX3CL1/fractalkine (1nM) and simultaneously stained with the high-affinity reporter mAb 327C for 10 minutes at 37°C. Cells were then analyzed by flow cytometry. The ratio between the mean fluorescence intensity of stimulated (open profile) versus unstimulated (black profile) cells is shown. The results shown for healthy control are representative of 6 individual experiments.$

The ability of chemokine to induce LFA-1 high-affinity state is impaired in NK cells from patients with WAS. Freshly isolated peripheral blood NK cells from healthy donors or patients with WAS were left untreated or stimulated with CXCL12/SDF-1 (10nM) or CX3CL1/fractalkine (1nM) and simultaneously stained with the high-affinity reporter mAb 327C for 10 minutes at 37°C. Cells were then analyzed by flow cytometry. The ratio between the mean fluorescence intensity of stimulated (open profile) versus unstimulated (black profile) cells is shown. The results shown for healthy control are representative of 6 individual experiments.

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