STAT5-induced genes can be divided as GATA1-dependent and GATA1-independent. (A) CB CD34⁺ cells, transduced with 4-OHT–inducible STAT5 and lentiviral vectors expressing short hairpins against luciferase and GATA1 were cultured for 3 days, STAT5 was induced by 4-OHT stimulation for 24 hours, and RNA was extracted. Equal amounts of RNA from 3 independent performed experiments were pooled and hybridized to Illumina arrays. Supervised cluster analysis: left panel, GATA1 targets with more then 2-fold difference; middle panel, STAT5 target genes in luciferase shRNA-transduced samples; and right panel, STAT5 target genes in GATA1 shRNA-transduced samples. (B) Venn diagram showing differences and overlap between the 3 groups analyzed in panel A. (C) Array-derived gene expression data of known erythroid-associated genes. (D) Array-derived gene expression data of several previously described STAT5 target genes, divided as GATA1-dependent and GATA1-independent STAT5 target genes. (E) Quantitative RT-PCR verification of several genes shown in panels C and D.