American Society of Hematology

Figure 1

$Figure 1. c-Myb is essential for B-cell development. (A-B) Flow cytometry of bone marrow of 3- to 4-week-old c-Myb+/+, c-MybPlt4/+, c-MybPlt4/Plt4, and c-MybΔmb1/Δmb1 mice. Numbers adjacent to the boxes indicate proportion of (A) total B cells (B220+CD19+) and (B) immature (B220loIgM+) and mature (B220hiIgM+) B cells among all live cells (mean ± SD of 4-10 mice per genotype). (C) Flow cytometry of density-fractionated and lineage-depleted bone marrow of 6- to 9-week-old c-Myb+/+, c-MybPlt4/+, c-MybPlt4/Plt4, and c-MybΔmb1/Δmb1 mice. CLPs were identified as Sca-1+Thy1− c-kitint IL-7Rα+; the gating strategy is available in supplemental Figures 1 and 3. Numbers in plots indicate the proportion of cells in each gated area (mean ± SD of 5-6 mice per genotype). (D-E) Flow cytometry of bone marrow of 3- to 4-week-old c-Myb+/+, c-MybPlt4/+, c-MybPlt4/Plt4, and c-MybΔmb1/Δmb1 mice. (D) Pre-pro-B cells were identified as CD19− CD11c− NK1.1− B220+CD43lo; the gating strategy is available in supplemental Figures 1 and 3. Numbers in plots indicate the proportion of cells in each gated area (mean ± SD of 4-10 mice per genotype). (E) The numbers above the boxes in the top plots indicate the proportion of pro-B cells (B220+c-kit+) among all live cells. The numbers above the boxes in the bottom plots indicate the proportion of committed B cells (CD19+) among B220+c-kit+ cells. Data are the mean ± SD of 4 to 10 mice per genotype. *P < .05; **P < .01; ***P < .001 in test group vs c-Myb+/+ cells.$

c-Myb is essential for B-cell development. (A-B) Flow cytometry of bone marrow of 3- to 4-week-old c-Myb^+/+, c-Myb^Plt4/+, c-Myb^Plt4/Plt4, and c-Myb^Δmb1/Δmb1 mice. Numbers adjacent to the boxes indicate proportion of (A) total B cells (B220⁺CD19⁺) and (B) immature (B220^loIgM⁺) and mature (B220^hiIgM⁺) B cells among all live cells (mean ± SD of 4-10 mice per genotype). (C) Flow cytometry of density-fractionated and lineage-depleted bone marrow of 6- to 9-week-old c-Myb^+/+, c-Myb^Plt4/+, c-Myb^Plt4/Plt4, and c-Myb^Δmb1/Δmb1 mice. CLPs were identified as Sca-1⁺Thy1⁻ c-kit^int IL-7Rα⁺; the gating strategy is available in supplemental Figures 1 and 3. Numbers in plots indicate the proportion of cells in each gated area (mean ± SD of 5-6 mice per genotype). (D-E) Flow cytometry of bone marrow of 3- to 4-week-old c-Myb^+/+, c-Myb^Plt4/+, c-Myb^Plt4/Plt4, and c-Myb^Δmb1/Δmb1 mice. (D) Pre-pro-B cells were identified as CD19⁻ CD11c⁻ NK1.1⁻ B220⁺CD43^lo; the gating strategy is available in supplemental Figures 1 and 3. Numbers in plots indicate the proportion of cells in each gated area (mean ± SD of 4-10 mice per genotype). (E) The numbers above the boxes in the top plots indicate the proportion of pro-B cells (B220⁺c-kit⁺) among all live cells. The numbers above the boxes in the bottom plots indicate the proportion of committed B cells (CD19⁺) among B220⁺c-kit⁺ cells. Data are the mean ± SD of 4 to 10 mice per genotype. *P < .05; **P < .01; ***P < .001 in test group vs c-Myb^+/+ cells.

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