CFU-S numbers are elevated in Slug−/− mice. (A) The frequencies of LSK cells as a percentage of total spleen mononuclear cells in Slug+/+ mice, Slug+/− mice, and Slug−/− mice. (B) Numbers of BM-derived and spleen-derived day 16 CFU-S colonies in Slug+/+, Slug+/−, and Slug−/− mice. For CFU assays, BM cells or spleen cells (8 × 104) were injected into lethally irradiated mice. Data are mean ± SD (n = 3 per group). #P > .05, *P < .05, and **P < .01 (Slug−/− or Slug+/− vs Slug+/+).