Figure 7
Interaction between IVIg and low-affinity FcγR is required for the inhibition of OVA-IC presentation. (A) Biotin-OVA-IC (1.66 μg/mL) was incubated with IFN-γ-activated P388D1 cells (1 × 105 cells) for 1 hour at 4°C in the presence or not of IVIg (10 mg/mL) or 2.4G2 mAb (30 μg/mL). Binding of biotin–OVA-ICs was monitored by flow cytometry using allophycocyanin-conjugated streptavidin. The MFIs are indicated in the histograms. (B) IVIg and F(ab′)2 fragments of IVIg were used at 10 mg/mL in the OVA-IC presentation assay. IL-2 secretion by DO-11.10 cells was measured as before. Results are representative of 3 independent experiments. *P < .001.
