Hematopoiesis with multilineage potential in the kidney and blood of adult runx1^W84X/W84X zebrafish. (A) Histologic sections of kidneys from WT and runx1^W84X/W84X zebrafish. Red arrows indicate hematopoietic cells; and yellow arrows, kidney tubules. The pictures are representative of kidneys from 10 each of the WT and runx1^W84X/W84X fish (original magnification ×400). (B) Flow cytometric analysis of kidney cells. FSC-A indicates forward scatter; and SSC-A, side scatter. Cell populations labeled with red circles are erythroid, blue for lymphoid, green for progenitors, and brown for myeloid. The numbers next to the circles indicate percentages for each cell population. The panels are representative of kidneys from 3 each of WT and runx1^W84X/W84X fish. (C) Peripheral blood cells in the WT and runx1^W84X/W84X zebrafish. Red arrows indicate erythrocytes; brown arrows, monocytes; blue arrows, lymphocytes; purple arrow, thrombocytes; green arrow, progenitor cell. Original magnifications ×100.