Expression of Fpn(D157G)-GFP in zebrafish results in embryonic iron deficiency. (A) Zebrafish embryos were injected with plasmid containing wild-type Fpn-GFP. (B) Zebrafish embryos were injected with plasmid containing Fpn(D157G)-GFP. At 24 hours postfertilization (hpf), embryo development was analyzed using an Olympus Microscope U-CMAD-Z camera and a 10× objective. (C) Zebrafish embryos were injected with Fpn(D157G)-GFP and with iron-dextran. At 24 hpf, embryos development was analyzed as in panel A. The figures are representative of 5 different experiments in which 200 embryos were injected for each condition. (D) Embryos injected with Fpn(D157G)-GFP with or without iron-dextran were solubilized in lysis buffer, and Fpn-GFP levels were assayed by Western blot analysis.