Pim2 expression in AML bone marrow. (A) Expression of Pim2 in AML according to karyotype. Box plots show the range of Pim2 expression between different AML karyotypes. Data were obtained from Affymetrix gene arrays performed on 220 primary AML samples.³³  (Normal/intermediate [n = 123]; poor risk [n = 39]; translocations t(8;21) and inv(16) [n = 41]; and APL with t(15;17) [n = 17].) The significance was calculated between groups using univariate 1-way analysis of variance test (P < .001). The median expression is shown by the horizontal line, the size of the box represents the borders that contain 50% of the values, and the error bars represent the upper and the lower quartile of the values. (B) Pim2 expression in primary human APL samples (n = 53), normal CD34⁺ cells (n = 7), and differentiated cells (normal granulocytes, monocytes, and lymphocytes; n = 6) analyzed by real-time RT-PCR. The expression levels of Pim2 were calculated by ddCt method using GAPDH as an internal control. The horizontal line represents the median.