MVs can integrate into various target cells. (A) Freshly isolated primary CLL B cells were incubated with MVs isolated from different CLL patients for 48 hours. Transfer of CD61 marker from MV to CLL B cells was analyzed by flow cytometry. (B) Purified MVs were labeled with the membrane dye PKH67 (green) and incubated with CLL-BMSCs for the indicated periods. Binding and internalization of MVs into BMSCs was visualized after staining with DAPI (nuclear stain) under confocal microscope (magnification level is indicated by the horizontal bar). (C) MV-mediated transfer of message into BMSCs was examined by Western blot after a 24-hour incubation by the use of an antibody to CD31. Expression of CD31 on MVs was verified by flow cytometry.