MVs induce expression of VEGF in CLL-BMSCs. (A) Production of total VEGF in the conditioned media of BMSCs stimulated with MVs as described previously was measured by ELISA and mean values are presented with SDs (pg/mL per 105 cells). *Statistical significance (P < .001) compared with the unstimulated controls. (B) CLL-BMSCs were exposed to MVs with or without presence of wortmannin or rapamycin for 24 hours. Cell lysates were analyzed for the expression of different isoforms of VEGF by Western blot by the use of an antibody to human VEGF. Results indicate that MVs induce expression predominantly of the VEGF165 isoform in CLL-BMSC. Actin was used as loading control. (C) Production of the antiangiogenic isoform VEGF165b in the aforementioned (B) conditioned media of BMSCs stimulated with MVs was measured by ELISA and mean values are presented (pg/mL per 105 cells).
