Transplantation experiments. (A) Kaplan-Meier survival curve of animals transplanted with hematopoietic precursors transduced with a HOXA gene and Meis1 as indicated (n = 5 for each cohort of animals). The figure depicts the state of the experiment at 140 days after transplantation. (B) WBC counts in peripheral blood of surviving animals from panel A (transduced with HOXA/Meis1) at 140 days after transplant. Gray boxes indicate individuals with elevated WBC numbers (>10 000 cells/μL) in each group. Control animals were age-matched nontransplanted ones. (C) FACS analysis of bone marrow and spleen cells from euthanized animals. Cells were double-stained as noted either for c-Kit and Gr-1 as precursor/granulocytic markers or with B220/CD3 to detect lymphoid cells. Each set of panels shows a representative sample of a single animal. (D) Peripheral blood smears stained with May-Grünwald-Giemsa. Blood was taken from the same animal as analyzed in panel C, stained, and microphotographs were taken at 2 different magnifications as described for Figure 4.