Augmented coagulation in TMpro mice during endotoxemia. (A) Western blot analysis confirming TM levels in lung protein samples from TMpro mice and wild-type mice (both 4-5 months old) that were sacrificed 6 hours after LPS injection (5 mg/kg, intraperitoneal). Control mice received no LPS (indicated as LPS−). Each lane contained 40 μg of protein from an individual mouse. (B) Desitometric analysis of panel A. ***Statistical significance (P < .001). (C) Western blot analysis demonstrating fibrin formation in kidney and liver from the same animals. Each lane contained 40 μg of protein from an individual mouse. (D) Densitometric analysis of panel C. Data are mean ± SD. *Statistical significance (P < .05). **Statistical significance (P < .01). This experiment was repeated once with similar results.
