Terminal erythroid and megakaryocytic differentiation. (A-B) Analysis of erythroid differentiation. CD36 and GPA expression was analyzed by flow cytometry before (E0) and 3 days (E3) or 6 days (E6) after induction of erythroid differentiation of shControl- or shGfi-1B–transduced cells. Numbers in plots indicate percentage of cells within each quadrant (A). Cytology of the same cells was analyzed after May-Grunwald-Giemsa staining (B). (C-D) Analysis of megakaryocytic differentiation. Infected cells cultured in the presence of TPO and SCF were observed (C) under an Eclipse TE3200 inverted microscope (Nikon) with a 40× oil objective. Images were collected with a cooled charge-coupled device camera (CoolSNAPfx; Roper Scientific) and the Metavue Imaging system (Universal Imaging) and then analyzed with ImageJ software (National Institutes of Health), and mature megakaryocytes were counted after May-Grunwald-Giemsa staining (D). All these results are representative of more than 4 independent experiments.