Kinetic of changes in IL-8 mRNA expression by neutrophils, in response to TLR stimulation. Control and mobilized neutrophils were incubated over time with LPS/LBP (A) or with MALP-2 (B). At various time points samples were taken and lysed with TRIzol. The expression of mRNA for IL-8 was assessed by quantitative RT-PCR. Results represent the data from 3 different experiments, with cells from 3 different donors. Data were analyzed by independent 2-way analysis of variance test; *P < .05, **P < .01 (significant difference).