Evolution of memory phenotypes of EBV-specific T cells after T-cell transfer. Peripheral blood cells from patient 1 (A) and patient 2 (B) were costained with HLA/peptide pentamer and monoclonal antibodies for CD8, CCR7, and CD45RA. CD8⁺ pentamer⁺ cells were gated as shown and analyzed for their expression of memory differentiation markers CCR7 and CD45RA. For each patient, T cells specific for 1 exemplary lytic cycle epitope (GLC or YVL, respectively) and for 1 exemplary latent antigen–derived epitope (CLG or RRIY) are shown. Expression pattern of CCR7 and CD45RA on total CD8⁺ T cells is shown in the rightmost column. Analyses of T cells from an early, an intermediate, and a late time point after T-cell transfer are shown.