Flubendazole induces cell death in malignant cell lines. (A) OCI-AML2 cells were treated for 72 hours with increasing concentrations of benzimidazoles. After incubation, cell growth and viability were measured by the MTS assay. Data are the mean percentage of viable cells ± SD from a representative experiment. (B) Leukemia and myeloma cell lines were treated with increasing concentrations of flubendazole. Seventy-two hours after incubation, cell growth and viability were measured by the MTS assay. Data are the mean percentage of viable cells ± SD from representative experiments. (C) Primary AML patient samples or (D) primary normal hematopoietic cells (1 × 105 cells; n = 3) were plated in a methylcellulose colony-forming assay with increasing concentrations of flubendazole. Colonies were counted 7 days after plating, as described in the supplemental Methods. Data are the mean ± SD from 3 independent experiments performed in duplicate.
