Cytosolic and membrane material internalization from live donor cells into vesicles by DCs in vitro. (A) DCs (transmission microscopy) were cultured with L cells previously stained with FDA (green, i; time-lapse confocal microscopy) and PKH 26 (red, ii). Capture of PKH 26 by DCs was represented as the MFI of PKH 26 per pixel in a single confocal plane every minute (iii). Pictures were taken from 71 minutes after the beginning of the culture. Results are representative of 2 independent experiments (original magnification ×63). (B-C) DCs (transmission microscopy) were cultured for 60 minutes with L cells transiently transfected with GFP-F (green) and stained with calcein orange CMRA (red). Results are representative of 2 independent experiments (original magnification ×160).