Clonal relationship of JAK2 wild-type leukemia to JAK2-mutant MPN with the use of 20q deletion or mutation in TET2 as a clonal marker. (A) Analysis of patient 8 showing a deletion of alternative 20q alleles in the MPN clone but no 20q deletion in the AML clone and a mutation of TET2 in leukemic blasts and a JAK2 wild-type erythroid colony but not in JAK2-mutant erythroid colonies. (B) Analysis of individual erythroid colonies from a patient harboring JAK2 V617F and MPL W515L mutations, showing that mutations in JAK2 and MPL are present in separate clones, with both clones have arisen from a shared TET2-mutant founder clone. (C) Analysis of patient 15 showing a mutation of TET2 in the JAK2 wild-type leukemia but not the JAK2-mutant MPN.