The Y7F CLEC-2 mutant inhibits signaling by the wild-type receptor. DT40 cells were transfected with 10 μg/mL of wild-type CLEC-2 and/or various amounts of Y7F CLEC-2 and an NFAT-luciferase reporter gene. Transfected cells were stimulated with 50nM rhodocytin for 6 hours at 37°C, after which time the amount of luciferase activity was measured as a readout of signaling. Results were normalized for transfection efficiency and plotted as a percentage of the wild-type response. Error bars represent the geometric mean ± SE of 3 to 8 separate experiments.