Electron microscopy and immunogold staining of CLEC-2 on resting and rhodocytin-stimulated mouse platelets. (Ai,Bi) Electron micrograph showing α-CLEC2 immunostaining. The majority of CLEC-2 immunogold labeling can be found within a pair (solid arrows) or alone (open arrowheads) under basal conditions (A) or as larger clusters after rhodocytin stimulation and rhodocytin immunogold labeling (B). Scale bar represents 50 nm. (Aii,Bii) Quantification of the CLEC-2 immunogold labeling distribution under basal (A) or rhodocytin-stimulated (B) conditions. The distribution of CLEC-2 immunogold labeling is depicted as a histogram. Data represent the mean plus or minus SD (n = 5). (Aiii) Quantification of the distance between CLEC-2 immunogold-labeled pairs. The intercenter distance between the gold particles within pairs was measured using ImageJ (n = 100) and presented as a box-and-whisker plot. Solid horizontal line shows the median for the data, the top of the box the 25th percentile, the bottom the 75th percentile, and the additional lines the range of the data.