Fluid shear stress induced phosphorylation-dependent HDAC5 nuclear export. (A-B) HUVECs were infected with Ad-GFP-HDAC5 or Ad-GFP-HDAC5-S/A. Twenty-four hours later, cells were conditioned with flow (left lane) or static (right lane) for the time indicated in each image. Images were captured at a magnification of ×60, using a fluorescence microscope.