HDAC5 down-regulated flow-mediated eNOS expression. (A-B) HUVECs were exposed to 8 or 24 hours of flow or to static conditions 24 hours after infection with Ad-HDAC5-S/A or Ad-LacZ (control). eNOS mRNA levels were assayed by real-time RT-PCR (A) and protein levels were detected by eNOS antibody (B). Vertical lines have been inserted to indicate repositioned gel lanes. *P < .05 compared with control (static). #P < .05 compared with that treated with flow + Ad-LacZ. Error bars represent ±SEM.