HDAC5-dependent pathway modulated flow anti-inflammation in endothelial cells. (A-B) Overexpression of Ad-HDAC5-S/A attenuated flow anti-inflammation. HUVECs were infected with Ad-HDAC5-S/A or Ad-LacZ as control. Twenty-four hours later, cells were subjected to flow or to static conditions for 24 hours followed by the treatment of TNFα or vehicle for 6 hours. U937 monocytes were added to HUVECs and incubated for 30 minutes. HUVECs were washed 3 times. The adherent monocytes were counted in 5 randomly optical fields in each dish. *P < .05, flow + TNFα + Ad-HDAC5-S/A vs flow + TNFα + Ad-LacZ. Error bars represent ±SEM.