Selective impairment of CD8αα TCRαβ IEL development in c-myc^ΔCD4 mice. (A) IELs from WT and c-myc^ΔCD4 mice were stained with TCRγδ, TCRαβ, CD4, CD8α, and CD8β. Dot plots represent staining of TCRγδ versus TCRαβ in total IELs, and CD4 versus CD8α and CD8β versus CD8α in the TCRαβ⁺ IEL population. Numbers in the dot plots indicate the percentage of cells represented in the quadrant. The charts below the dot plots represent the absolute numbers of the indicated IEL subsets in individual mice (○ represents WT mice; and ●, c-myc^ΔCD4 mice). Horizontal line in each group represents the mean value. Statistically significant differences: *P < .05, ***P < .001. (B) cDNA from the indicated sorted populations was assayed for c-myc expression by quantitative PCR. c-myc levels were normalized to TBP and presented in arbitrary units. Error bars indicate mean ± SD.