c-Myc deficiency affects IL-15R expression, proliferation, and survival of CD8αα TCRαβ IELs. (A) Expression of IL-15R chains on CD8αα and CD8αβ TCRαβ IEL subsets from WT and c-myc^ΔCD4 mice. The number in each histogram corresponds to MFI of the IL-15R staining (empty histogram) minus MFI of the unstained control (gray filled histogram) (mean ± SD; n = 4). (B) Cell-cycle analysis of TCRαβ IELs in WT and c-myc^ΔCD4 mice. Dot plots show Ki67 versus Hoechst profile for CD8αβ and CD8αα TCRαβ IELs. Numbers in dot plots correspond to percentages of cells in G₀, G₁, and S + G₂/M. Results are representative of 2 independent analyses. (C) Bar graph represents the percentage of apoptotic (annexin V⁺ 7-amino-actinomycin D⁻) CD8αβ and CD8αα TCRαβ IELs in WT and c-myc^ΔCD4 mice (mean ± SD; n = 6). *Statistically significant difference (P < .05).