CD8αα TCRαβ IEL development in c-myc^ΔCD4 mice is partially restored by enforced BCL-2 expression. (A) Intracellular mouse Bcl-2 expression in the indicated populations from WT and c-myc^ΔCD4 mice. Dashed histogram represents the staining with IgG control. Bar graph represents MFI of mouse Bcl-2 staining (mean ± SD; n = 6). *P < .05. (B) Intracellular mouse Bcl-2 or human BCL-2 expression in CD8αα TCRαβ IELs from WT, BCL-2 Tg, c-myc^ΔCD4, and c-myc^ΔCD4 BCL-2 Tg mice. The number in each histogram indicates the MFI of Bcl-2 staining. (C) Bar graph represents the absolute number of cells in the indicated IEL subsets in WT, BCL-2 Tg, c-myc^ΔCD4, and c-myc^ΔCD4 BCL-2 Tg mice (mean ± SD; n = 5). *Statistically significant difference (P < .05). (D) Thymic Vα14i NKT cells and memory phenotype CD8 splenic T cells in WT, BCL-2 Tg, c-myc^ΔCD4, and c-myc^ΔCD4 BCL-2 Tg mice. CD8-depleted thymocytes were stained with TCRβ and CD1d-dimer. Percentage of Vα14i NKT cells (TCRβ⁺ CD1d-dimer⁺) is indicated in the upper dot plots. Total spleen cell suspensions were stained with CD8α, CD62L, CD122, and CD44. Lower dot plots represent CD122 versus CD44 profile of the CD8α⁺ CD62L⁺ splenic T cells. The percentage of memory phenotype T cells (CD122⁺ CD44⁺) is indicated. Data are representative of 3 independent experiments.