Normal development of unconventional T cells in c-myc^N/N mice. (A) cDNA from the indicated populations from control (white bars) and c-myc^N/N (black bars) mice was assayed for c-myc and N-myc expression by quantitative RT-PCR. Myc levels were normalized to TBP and are presented in arbitrary units. (B) Flow cytometric analysis of IELs from control and c-myc^N/N mice. Samples were stained and analyzed as in Figure 1. Numbers indicate the percentage of cells in each quadrant. Bar graphs represent absolute numbers (mean ± SD; n = 5) of the indicated IEL subsets. (C) Thymic Vα14i NKT cells and splenic memory phenotype CD8⁺ T cells in WT and c-myc^N/N mice. Samples were stained and analyzed as in Figure 5D. Data are representative of 2 independent experiments.