VWF-platelet binding assay to examine the enhanced binding of recombinant mouse type 2B VWF variants. Recombinant mVWF was incubated with formalin-fixed mouse platelets in the presence or absence of botrocetin. The amount of bound VWF is expressed as a percentage of a control incubation that was composed of mVWF in the absence of platelets and botrocetin. Data are mean ± SEM of 6 experiments.