Analysis of recombinant mouse ADAMTS13 digestion of recombinant mouse VWF. A total of 1 U/mL mVWF was digested with various concentrations of recombinant mADAMTS13 for 24 hours at 37°C. Distances were graphed using a 4-parameter curve, and the concentration of mADAMTS13 required to cause loss of 50% of multimer height was determined. Data are mean ± SEM of 2 experiments.