Effect of CTX on the absolute and relative numbers of different DC subsets in lymph nodes. Mice were treated with CTX (150 mg/kg) by intraperitoneal injection and killed 2, 4, 7, and 16 days after drug administration. Subcutaneous LNs and spleen were harvested, digested, and assessed for the number and proportion of skin-derived migratory DCs (CD11c^int, MHC II^high), resident DCs (CD11c^high, MHC^int), or pDCs (CD11c^int, MHC^low, B220⁺, Gr1⁺) at the time points indicated and compared with untreated naive controls. (A) DC subsets in subcutaneous LNs and spleen of naive mice stained for CD4, CD8, and (B) CD86, CD11b, and CD205. Representative plots of naive LN DCs and LN DCs 4 days after CTX treatment (C). The proportions of skin-derived DCs, resident DCs, and pDCs in the LNs as measured by flow cytometry at various time points after the administration of CTX (D). Absolute cell number of different DC subsets was also counted using the total cell count of LN cells. Fold decrease in absolute number of LN DC subsets 4 days after CTX treatment (E). CD8 indicates CD8⁺ resident DCs; CD4, CD4⁺ resident DCs; DN, CD8⁻CD4⁻ resident DCs; and pDC, plasmacytoid DCs. Data are representative of 4 independent experiments. *Statistically significant.