17-DMAG down-regulates NF-κB signaling through IKKα and IKKβ. Western blot analysis for AKT, IKKα, and IKKβ in whole cell lysate prepared from CLL patient cells treated with vehicle control or increasing doses of 17-DMAG for 24 hours. Blots are probed with actin as a loading control. Results shown are representative of 8 patient samples.