DcTs mitigate GVHD. (A) Schema of the experiments. 9 Gy total-body irradiation was used to condition recipient mice (Balb/c). B6 mice (CD45.2) TCD BM (5 × 10⁶ cells) were used as a stem cell source. To induce GVHD, 5 × 10⁵ B6 Tconv (CD45.1) was infused along with donor TCD BM. To test suppressor function of dcTs, 5 × 10⁵ B6 CD4⁺CD25⁺ nTregs (for control), pbsTs or dcTs (all CD45.2) generated from CD4⁺CD25⁻ T cells were injected with TCD BM and Tconv. (B-D) Mice that underwent a transplantation with dcTs show significantly higher survival rate (B) and less weight loss (C) than mice infused with pbsTs and a trend toward increase of B cells (D) analyzed 1 month after transplantation. A pool of 2 independent experiments. (E) Schema of the experiments. B6 mice (CD45.1) TCD BM (5 × 10⁶ cells) were used as a stem cell source. A total of 10 × 10⁶ pbsTs/dcTs generated from Tconv (B6, CD45.2) were given. (F-G) Mice that underwent a transplantation with dcTs show significantly higher survival rate (F) and more B cells (G) than mice that received pbsTs. A pool of 3 independent experiments analyzed 1 month after transplantation.