Impaired CR in FXIII-A-KO mice but normal CR in tTG KO mice. (A) Photograph of CR in wild-type mice (left), tTG KO mice (middle), FXIII-A KO mice (right). PRP was incubated with 1 U/mL thrombin, 5mM CaCl2 at 37°C. The photograph was taken after 1 hour. (B) Quantitative analysis of CR by a weight ratio method. The extent of CR was assessed at 10 and 60 minutes by measuring the clot weight, for wild-type (open), tTG KO (shaded), or FXIII-A KO (filled) mice. Data are mean ± SD of triplicates. (C) Time-dependent CR measured by an area ratio method. The extent of CR was assessed at the indicated times by measuring the clot size, for wild-type (●), tTG KO (■), or FXIII-A KO (▴) mice. Data are mean ± SD of triplicates. *Statistically significant differences (P < .001) were observed at 10 and 60 minutes in panel B and at 15, 30, 45, 60, 75, and 1440 minutes in panel C between FXIII-A-KO versus wild-type mice.
