Effects of ODN on the growth of hematopoietic progenitors derived from CD34+ cells. (A) CD34+ cells were cultured with IL-3, SCF, EPO, G-CSF, TPO, and various concentrations of 2006-PO ranging from 0 to 4μM. Seven days later, the total cell yield was counted and GPA, CD15, and CD61 expression examined using fluorocytometry. Data are mean ± minus SD of 3 independent experiments. (B-D) CD34+ cells were cultured with IL-3 and SCF in the presence of EPO at a cell density of 2 × 104 cells/mL (B), with G-CSF at a cell density of 3 × 105 cells/mL (C), or with TPO at a cell density of 3 × 105 cells/mL (D) with or without 2006-PO. Seven days later, the cell yields were calculated as a percentage relative to the total number of cells without 2006-PO. Data are mean ± SD of 3 independent experiments. The absolute number of cells generated from CD34+ cells without 2006-PO was 7.6 ± 2.2 × 105 cells (A), 8.6 ± 3.6 × 105 cells (B), 4.0 ± 0.6 × 105 cells (C), and 4.0 ± 0.6 × 105 cells (D). (E) CD34+ cells were cultured in erythroid medium in the presence or absence of various forms of ODN at 4μM. The total cell yield was then calculated as a percentage relative to the total number of cells without ODN (5.3 ± 1.4 × 105 cells/2 × 104 CD34+ cells cultured; n = 4). Data are mean ± SD of 4 independent experiments. *P < .05. ***P < .001. NS indicates no significance.
