Consensus sequence with 2006-PO in human parvovirus B19 genome (453 5′-GTTTTGT-3′ 459) inhibits erythroid growth. (A) Map of the B19 genome, highlighting the region that includes the consensus sequence (5′-GTTTTGT-3′) and the sequences of synthetic ODN. (B) Effects of synthetic B19 ODN with PO backbone on erythroid growth. Purified CD34⁺ cells were cultured in erythroid medium, with or without ODN. After 7 days in culture, the generated cells were collected, washed, and counted. The cell yields are presented as a percentage relative to the total number of cells without ODN. Data are mean ± SD of 3 independent experiments. The total cells generated from 2 × 10⁴ CD34⁺ cells were 4.3 ± 0.8 × 10⁵ cells. (C) Effects of ODN 5′-GTTTTGT-3′ and its complementary ODN 5′-ACAAAAC-3′ (top panel), and 5′-TTTTGT-3′ (bottom panel) with PO backbone on erythroid growth. Purified CD34⁺ cells were cultured in erythroid medium, with or without ODN. After 7 days in culture, the generated cells were collected, washed, and counted. The total cell yields are presented as a percentage relative to the total number of cells without ODN. Data are mean ± SD of 3 independent experiments. The absolute number of cells generated from 2 × 10⁴ CD34⁺ cells was 5.4 ± 2.1 × 10⁵ cells (top panel) and 6.8 ± 0.8 × 10⁵ cells (bottom panel). *P < .05. ***P < .001. NS indicates no significance.