Inhibition of VEGF receptors in zebrafish results in reduced etsrp⁺ angioblast numbers including reduced numbers at the midline. Embryos were treated from 10 to 16 hpf with vehicle control DMSO (A) or VEGF receptor inhibitors ZM323881 (1 μm; B) or SU4516 (10 μm; C), and angioblast positions were analyzed by whole-mount ISH for etsrp. Inhibitor-treated embryos show reduced angioblast numbers, and strongly reduced etsrp⁺ cells at the midline. (D) ecscr morphants show disrupted anterior vascular cord. Each result is representative of more than 20 embryos from 2 independent experiments. Scale bar in panel A represents 200 μm.