American Society of Hematology

Figure 2

$Figure 2. Concurrent deletion of Smad1 and Smad5 alters the colony-forming ability of myeloid progenitors in vitro. (A) Quantity of phenotypic hematopoietic stem and progenitor cells in WT and S1/5−/− mice, as determined by differential counts and FACS (LT-HSC; LSKCD34−Flt3low, ST-HSC; LSKCD34+Flt3low, lymphoid-primed multipotent progenitor; LSKCD34+Flt3high). (B) Relative fraction of dividing cells as defined by expression of Ki67 in LT-HSCs (LSKCD34−) and multipotent progenitors (LSKCD34+). Representative experiment using BM from one S1/5−/− mouse and a littermate control (left), and pooled data from 3 independent experiments (right). (C) Single-cell proliferation culture of primitive LSKCD34− cells, and (D) bulk culture of c-kit-enriched BM cells. (E) Quantity of myeloid progenitors, as determined by the number of colony-forming cells (GM-CFU). Data are mean ± SD (n = 2-5). *P < .05. represent WT; and ▬, S1/5−/−.$

Concurrent deletion of Smad1 and Smad5 alters the colony-forming ability of myeloid progenitors in vitro. (A) Quantity of phenotypic hematopoietic stem and progenitor cells in WT and S1/5^−/− mice, as determined by differential counts and FACS (LT-HSC; LSKCD34⁻Flt3low, ST-HSC; LSKCD34⁺Flt3^low, lymphoid-primed multipotent progenitor; LSKCD34⁺Flt3^high). (B) Relative fraction of dividing cells as defined by expression of Ki67 in LT-HSCs (LSKCD34⁻) and multipotent progenitors (LSKCD34⁺). Representative experiment using BM from one S1/5^−/− mouse and a littermate control (left), and pooled data from 3 independent experiments (right). (C) Single-cell proliferation culture of primitive LSKCD34⁻ cells, and (D) bulk culture of c-kit-enriched BM cells. (E) Quantity of myeloid progenitors, as determined by the number of colony-forming cells (GM-CFU). Data are mean ± SD (n = 2-5). *P < .05. represent WT; and ▬, S1/5^−/−.

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