TaqMan qRT-PCR on MLL-r cell lines shows dose-dependent re-expression of genes of interest when treated with decitabine. TaqMan qRT-PCR was performed on cell lines treated with decitabine at concentrations of 0, 0.5, 1, 2, and 4μM. Copy number fold change was estimated using the ΔΔCt method. (A-C) In cell lines carrying MLL-r several of the genes of interest showed robust dose-dependent increase in copy number, whereas this phenomenon was not seen in the MLL-wt cell lines (D-F). This demonstrates biologic correlation with the MTT assays.