Visualization of KIR3DL2-ODN interaction in NK cells. Confocal microscopy analysis of NK cells untreated (A) or incubated with 5 μg of TxRed-ODN (red) for 30 minutes at 4°C (B), or for 15 or 30 minutes at 37°C (C-D). Cells were then stained for KIR3DL2, KIR2DL2/S2/L3, or CD56 (green), as indicated. Images are single confocal sections taken with a 60× objective. Scale bar represents 2 μm. Quantitative colocalization analysis was performed to compare panels C and D, and it provided the following Mander coefficients of colocalization: 0.9 ± 0.04 (C), 0.56 ± 0.07 (D); P < .001.