CD8 T cells fail to be tolerized in NFAT1^−/− mice. WT B6.129/F2 and NFAT1^−/− mice received 3 Gy TBI/anti-CD154 and B10.A BMT. For the groups depicted in panels C and D, recipient CD8 T cells were depleted on day −1. Donor cell chimerism in peripheral blood leukocytes was followed over time. (A,C) Incidence of chimerism is indicated for each group in different lineages. Chimerism among CD4 cells (not shown) was comparable with that of CD8 cells. A lineage was defined as chimeric when ≥ 5% donor cells were detected in that lineage. (B,D) Levels of chimerism in mice that showed engraftment at 2 weeks are depicted over time for the granulocyte and B-cell lineages. One representative experiment of 2 is shown (n = 6-7/group).